For in vitro diagnostic use. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 1, samples per run. All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results that are accurate and reproducible.
Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain publication-ready results. Versatile for experienced users. Take advantage of the first plate-based real-time qPCR instrument that provides 1, data points in a single PCR run in less than 50 minutes. A proven standard of excellence. It was the first system to introduce hybridization probes, true melting curve analysis, automated absolute quantification, relative quantification with efficiency correction, and more.
Designed to maximize the performance of your system. Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity and specificity for your real-time PCR assays. Choose the reagent that's best for your experiments on:. Non-Roche qPCR instruments. Bring your qPCR to the next level with the new 5x concentrated multiplex master mixes. Detect a greater number of targets per reaction, work more efficiently, and maximize your valuable samples. Sensitive, specific and one-vial EvoScript master mixes turn even the smallest clues into measurably better results, ensuring no evidence gets lost in transcription.
Not sure which kit is best for you? With the new Roche Reagent Selection guide, you can easily find which real-time PCR reagents are right for your applications. The Genomic Workflow Publication Database is your source for relevant, reliable information specific to your research applications. Easily design your qPCR assay to analyze over 5 million transcripts of any sequenced organism.
Function-tested qPCR single assays or custom panels, for human, mouse or rat targets of your choice. Choose from a variety of Roche probe-based qPCR assay solutions for your gene expression needs.
Opt for universal probes for affordable and easy-to-design assays. Or select function-tested single assays and customized panels to skip design and optimization steps. Choose the assay solution that's best for you. Ready to use assays designed for multiplexing. Choose from an extensive range of assays for pathogen detection. Compatibility with Roche reagents and instruments enables seamless assay integration into your workflow.
Experience how fast and easy it is to design a specific, cost-effective, hydrolysis probe-based qPCR assay in 3 easy steps using our free online software.
Skip time-consuming assay design and optimization steps with the function-tested RealTime ready qPCR assays for gene expression for human, mouse, rat or other species.
Receive ready-to-use probe-based assays, compatible with any real-time PCR instrument. All assays are function-tested and pre-plated for the upmost convenience and quick time to results. Designed to maximize performance. Currently, analysis of gene expression is a critically important area of scientific research. Many genomic labs have found that quantitative real-time PCR is the gold standard for fast, sensitive determination of gene expression levels.
However, not all quantitative real-time PCR techniques are appropriate for gene expression analysis. There are many types and subtypes of quantitative real-time PCR methods, each of which is characterized by its requirements, its complexity, and its reliability.
However, it is possible to group all these methods under two main analysis techniques - absolute and relative quantification. The technique you choose depends on the complexity of your analysis and the desired format of the final result:. It also enables normalization for run-to-run differences caused e.
Basic Approach - Easy, convenient, fast, this method works with pre-defined settings, requiring no user input. It is an automated analysis, with one click to results.
Advanced Approach - For highest flexibility and most demanding analyses, this approach provides an analysis with extended options according to the user's needs. Advanced features include the use of multiple housekeeping genes for pairing. Serially diluted standards dilutions, three replicates each were assayed along with unknown samples.
The final results from b are automatically calculated from the Cp values of the target unknowns and calibrator and the reference unknowns and calibrator ; these results are depicted in c.
Click here for RealTime ready pre-tested assays and customized panels. Online Technical Support Find help. Last Update On Products are for life science research only. Not for use in diagnostic procedures unless otherwise indicated.
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For life science research only. Not for use in diagnostic procedures. To order these products, please contact your Roche order management team. The amplification of a viral target in twofold dilutions ranging from 1x10 2 to 1x10 5 copies per reaction is shown.
The user can also choose which samples to include in the display and analysis e. The plate scheme indicates which samples are positive, negative, or uncertain, or whether they are standards.
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